Skinned fibers of frog semitendinosus muscle could easily be stretched up to 8 mum or more in sarcomere length. Such extremely stretched fibers gave quite sharp optical diffraction patterns. The intensities of all observable diffraction lines were found to increase on application of electric field (10 similar to 100 V/cm) parallel to the fiber axis, provided that there was no overlap between thin and thick filaments. By use of a polarizing microscope, it was concluded that I-bands were mainly responsible for this intensity increase. By application of square pulses, the time course of the intensity increase and decay was followed. The analysis based on a simple model suggests: (a) Each thin filament has a permanent dipole movement and the movement directs from Z-bands to the free end of the thin filament. (b) The flexural rigidity of thin filaments is estimated to be similar to 3 with 10-17 dyn with cm-2. The present fibers will provide various applications in physiochemical studies of in vivo thin and thick filaments.
展开▼
机译:蛙半腱肌的皮肤纤维很容易被拉长到肌节长度的8毫米或更多。这种极度拉伸的纤维给出了非常清晰的光学衍射图。发现在平行于纤维轴的电场(10个类似于100 V / cm)的电场作用下,所有可观察到的衍射线的强度都会增加,前提是细丝和粗丝之间没有重叠。通过使用偏光显微镜,可以得出结论,I波段是造成强度增加的主要原因。通过施加方脉冲,遵循强度增加和衰减的时间过程。基于简单模型的分析表明:(a)每个细丝都有一个永久的偶极子运动,并且该运动从Z波段直接指向细丝的自由端。 (b)细丝的抗弯刚度估计与cm-2为10-17 dyn的3相似。本发明的纤维将在体内细和粗丝的生理化学研究中提供各种应用。
展开▼